包核为H₂O₂的超声造影剂研究

包核为H 2 O 2 的超声造影剂研究 Study On New Ultrasonic Contrast Agent Encapsulating H 2 O 2 卓仁禧 ZHUO Ren\|xi 1 2 first-author 丁雄军 DING Xiong\|jun 1 2 傅功成 FU Gong\|cheng 1 2 李威 LI Wei 1 2 教育部生物医用高分子材料重点实验室!武汉大学化学与环境科学学院 教育部生物医用高分子材料重点实验室!武汉大学化学与环境科学学院 Key Laboratory of Biomedical Polymer Materials,the Ministry of Education Key Laboratory of Biomedical Polymer Materials,the Ministry of Education ,湖北,武汉,430072 ,湖北,武汉,430072 College of Chemistry and Environment Scince,Wuhan University, Wuhan 430072,Hubei,China) \ \ College of Chemistry and Environment Scince,Wuhan University, Wuhan 430072,Hubei,China) \ \ 以双氧水为包核 ,以卵磷脂 （ PC） ,二肉豆蔻酰磷脂酰胆碱 （ DPPC） ,司盘 6 0 （ Span 6 0 ） ,吐温 6 0 （ Tween6 0 ）等为膜材 ,用逆相蒸发法或超声法制备了 12种囊泡 ,其平均直径在 0 .1～ 3μm范围内 .研究了囊泡的包封率、形状以及尺寸大小随时间变化的情况 .实验结果表明采用逆相蒸发法制得的囊泡稳定性较好 ;而膜材不同 ,囊泡的稳定性也有明显的差别 .选取包封率较高的一种囊泡 ,在 37℃ ,过氧化氢酶存在下测定了 H2 O2 的释放速率 .将部分囊泡通过静脉注射到家兔体内 ,观察到其肝部位的超声波信号有明显增强 New microbubbles were prepared through two different methods by the use of H 2O 2 as the nuclei and PC,DPPC,Span 60 or Tween 60 as the encapsulating membrane. The size of the microbubbles ranged from 0.1 μm to 3 μm and encapsulating effectiveness reached 25% to 42%. nio2, which was prepared from Tween 60 exhibited better stability than that of the other microbubbles. Releasing rate of H 2O 2 from some of the microbubbles were determined at 37℃ in the presence of hydrogen peroxidase. The animal experiment results of ultrasonic imaging showed that some of the microbubbles could produce hepatic enhancement due to back scattering of O 2 which stemmed from enzymatic catalysis of H 2O 2. 超声造影剂 囊泡 过氧化氢 背向散射 ultrasonic contrast agent microbubble Hydrogen peroxide back scattering 10.14188/j.1671-8836.2000.06.001 TQ421 2000-06-01 2021-04-01 6