不同氮源对水稻幼苗根氨同化酶的影响

不同氮源对水稻幼苗根氨同化酶的影响 Effect of Different Nitrogen Sources on Ammonia-assimilating Enzymes of the Roots in Rice Seedlings 李泽松 LI Ze\|song 1 first-author 林清华 LIN Qing\|hua 1 张楚富 ZHANG Chu\|fu 1 彭进 PENG Jin 1 魏国威 WEI Guo\|wei 1 武汉大学植物发育生物学教育部重点实验室,湖北,武汉,430072 武汉大学植物发育生物学教育部重点实验室,湖北,武汉,430072 Key Laboratory of MOE for Plant Developmental Biology, Wuhan University Key Laboratory of MOE for Plant Developmental Biology, Wuhan University 将氮素含量相同、但来源不同的氮源分别加入到水稻培养液中 ,分析了不同氮源对水稻幼苗根氨同化酶活性的影响 .与无氮培养的水稻根相比 ,（ NH4） 2 SO4,NH4NO3,KNO3,谷氨酸 （ Glu）和谷氨酰胺 （ Gln）都能分别促进水稻根谷氨酰胺合成酶 （ GS）、依赖于 NADH的谷氨酸合酶 （ NADH- GOGAT）和依赖于 NADH的谷氨酸脱氢酶 （ NADH- GDH）活性 .（ NH4） 2 SO4对 GS和 NADH- GOGAT活性促进最为显著 ,而 Gln对 GS和 Glu对 NADH-GOGAT的活性分别影响最小 .Glu对 NADH- GDH活性的诱导稍强于其它氮源 .这些结果提示 ,不同氮源对水稻根氨同化酶活性影响的差别也许反映了这些物质对水稻根部氮素代谢的影响以及对这些酶诱导能力的不同 The same amount of nitrogen from the different sources was added to the nutrition solutions of rice plant growth, respectively. The effect of different nitrogen sources on ammonia\| assimilating enzymes of the roots in rice seedlings was studied. As compared with the roots of rice seedlings grown in N\|free nutrition solution, the activities of glutamine synthetase （GS）, NADH\|dependent glutamate synthase （NADH\|GOGAT）, and NADH\|dependent glutamate dehydrogenase （NADH\|GDH） were stimulated by （NH 4） 2SO 4, NH 4NO 3, KNO 3, glutamic acid （Glu）, and glutamine （Gln）. The stimulation of （NH 4） 2SO 4 for the activities of GS and NADH\|GOGAT was more remarkable, but the effect of Gln on GS as well as Glu on NADH\|GOGAT was little, respectively. The stimulation of Glu to NADH\|GDH was more obvious than other nitrogen sources. The results suggested that the different effect of these nitrogen sources on ammonia\|assimilating enzymes might reflect the discrepancy of the nitrogen metabolism and the distinction of the induced ability for these enzymes in roots of rice seedlings. 谷氨酰胺合成酶 谷氨酸合酶 谷氨酸脱氢酶 氮源 水稻 glutamine synthetase glutamate synthase glutamate dehydrogenase nitrogen source rice 10.14188/j.1671-8836.2000.06.020 Q945.1;Q946.5 国家自然科学基金资助项目!（ 3 9870 5 2 7） 2000-06-01 2021-04-01 6