克氏原螯虾（Procambarus clarkii）应急二价铜离子时超氧化物歧化酶和过氧化氢酶的变化

克氏原螯虾（Procambarus clarkii）应急二价铜离子时超氧化物歧化酶和过氧化氢酶的变化 Activity Change of Superoxide Dismutase and Catalase of Crayfish （Procambarus clarkii） under Copper Ion Stress 李忠 LI Zhong1 1 first-author 梁宏伟 LIANG Hongwei1 1 呼光富 HU Guangfu1 1 2 王长忠 WANG Changzhong1 1 2 吴勤超 WU Qinchao1 1 2 罗相忠 LUO Xiangzhong1 1 邹桂伟 ZOU Guiwei1 1 中国水产科学研究院长江水产研究所 中国水产科学研究院长江水产研究所 Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences 华中农业大学水产学院 华中农业大学水产学院 College of Fisheries,Huazhong Agricuhural University College of Fisheries,Huazhong Agricuhural University 用二价铜离子（Cu2+）胁迫克氏原螯虾,分析其肝胰腺、触角腺、鳃等组织的超氧化物歧化酶（superoxide dismutase,SOD）和过氧化氢酶（catalase,CAT）活性变化.结果表明:克氏原螯虾能在含10mg/LCu2+水中生活;在10mg/LCu2+胁迫下,在0～24h,肝胰腺和触角腺中的CAT、SOD活性均显著升高,CAT活性在24h达到最高;在24～36h,肝胰腺和触角腺中的CAT活性开始下降,而SOD活性继续上升,在第36h时达到最高;48h后,肝胰腺和触角腺中SOD和SOD活性下降并均趋于平稳表达,SOD活性仍显著高于非胁迫处理时,而CAT活性则显著低于Cu2+处理时;鳃中的SOD和SOD活性未受到Cu2+浓度变化的影响. Crayfish,（Procambarus clarkii） was treated with different concentrations of Cu2+,the activity of superoxide dismutase （SOD） and catalase （CAT） in hepatopancreas,antennary gland and gill measured. It was found that crayfish survived 10 mg/L Cu2+,in which the activity of SOD and CAT in hepatopancreas and antennary gland increased during 0-24 hour and the activity of CAT reach its max at 24 hour;after 24 hour,CAT in hepatopancreas and antennary gland gradually decreased,SOD still increased and reach it max level at 36th hour;after 48 hour SOD and CAT of hepatopancreas and antennary gland were decreased and reach a stable level,SOD activity were increase and CAT activity was decreased significantly compare with controlled;while the activity of SOD and CAT in gill showed no evident changes during the process of Cu2+ treatment. 克氏原螯虾 二价铜离子 超氧化物歧化酶 过氧化氢酶 crayfish bivalent copper ion superoxide dismutase catalase 10.14188/j.1671-8836.2010.01.016 S917.4 国家科技基础条件平台专项（2006DKA30470-002）;中国水产科学院长江水产研究所所长基金资助项目 2010-01-01 2021-04-01 1 56