修饰的Ac/Ds转座子元件在油菜中的遗传转化与鉴定

修饰的Ac/Ds转座子元件在油菜中的遗传转化与鉴定 Transformation and Identification of Modified Ac and Ds Transposon Elements in Brassica napus 徐娟 XU Juan1 1 2 first-author 闫晓红 YAN Xiaohong1 1 王力军 WANG Lijun1 1 黄进勇 HUANG Jinyong2 2 魏文辉 WEI Wenhui1 1 中国农业科学院油料作物研究所/农业部油料作物生物学重点开放实验室 中国农业科学院油料作物研究所/农业部油料作物生物学重点开放实验室 Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Oil Crop Biology of the Ministry of Agriculture Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Oil Crop Biology of the Ministry of Agriculture 郑州大学生物工程系 郑州大学生物工程系 Department of Bioengineering,Zhengzhou University Department of Bioengineering,Zhengzhou University 玉米Ac/Ds转座子系统已被成功应用于多种异源植物进行基因的分离与克隆.本研究利用农杆菌介导法将含有Ac元件的质粒pUbiAc及含有Ds元件的质粒pDsBar1300分别转化甘蓝型油菜中双6号的子叶柄与下胚轴,分别获得了Ac和Ds的转化植株.PCR检测发现,在10株Ac转化植株中有1株为阳性苗,阳性率为10%;在28株Ds转化植株中有21株阳性苗,阳性率为75%.该结果为下一步构建油菜突变体群体及开展油菜功能基因组学研究将开辟一个新的途径. Maize Ac/Ds transposon system has been successfully applied in a variety of heterologous plants to clone genes.The plasmid pUbiAc inserted Ac and plasmid pDsBar1300 inserted Ds were transformed into cotyledon petiole and hypocotyl of Brassica napus cultivar zhongshuang 6 by Agrobacterium Tumefaciens-mediated method.From the survived 10 Ac transgenic plants,1 positive transgenic individual was identified by PCR assay with a positive frequency of 10%.From the survived 28 Ds transgenic plants,21 positive transgenic individuals were identified by PCR with a positive frequency of 75%.This study will probably supply a new method for the construction of Brassica napus mutant plants and its functional genomics research. Ac/Ds转座子 甘蓝型油菜 转化 PCR鉴定 Ac/Ds transposon Brassica napus Transformation PCR identification 10.14188/j.1671-8836.2010.03.018 S565.4 国家自然科学基金（30671312）;湖北省自然科学基金（2008CDA083,2009CDB191）;中央级公益性科研院所基本科研业务费专项资金资助项目 2010-03-01 2021-04-01 3 56