凡纳滨对虾基因组fosmid文库的构建及钙离子通道基因的筛选

凡纳滨对虾基因组fosmid文库的构建及钙离子通道基因的筛选 Construction of a Fosmid Library of Litopenaeus vannamei and Screening of a Calcium-Release-Channel Gene 马宁 MA Ning 1 first-author 曾地刚 ZENG Digang 1 陈晓汉 CHEN Xiaohan 1 广西水产研究所 广西水产研究所 Fisheries Research Institute of Guangxi Fisheries Research Institute of Guangxi 本研究构建了凡纳滨对虾的基因组fosmid文库,该文库包含大约1.1×105个克隆,插入片段平均长度大于35 kb.根据和果蝇钙离子通道基因同源的凡纳滨对虾EST序列设计引物,对文库进行了PCR筛选,得到1个阳性克隆,测序结果显示该克隆包含有钙离子通道基因.研究结果为凡纳滨对虾的基因克隆和分子选育等研究奠定了基础. A fosmid genomic library was constructed for Litopenaeus vannamei.The library consisted of about 1.1×105 clones,in which average length of inserts was greater than 35 kb.The library was screened by PCR analysis using primers designed according to the EST from L.vannamei homology to calcium-release-channel gene of Drosophila melanogaster.A positive clone was selected,subjected to DNA sequencing,and an existence of the calcium-release-channel gene was revealed.These results will improve the study on gene cloning and molecular breeding for L.vannamei. 凡纳滨对虾 fosmid文库 钙离子通道基因 Litopenaeus vannamei fosmid library calcium-release-channel gene 10.14188/j.1671-8836.2010.03.019 Q78;S917.4 国家自然基金项目（30860214）;广西自然科学基金资助项目（0991106）;广西科技攻关项目（0992025-3） 2010-03-01 2021-04-01 3 56