利用SRAP和ISSR标记分析五节芒（Miscanthus floridulus）的遗传多样性

利用SRAP和ISSR标记分析五节芒（Miscanthus floridulus）的遗传多样性 Analysis of Genetic Diversity in Miscanthus floridulus Using SRAP and ISSR Markers 刁英 DIAO Ying1 1 2 first-author 胡小虎 HU Xiaohu1 1 郑兴飞 ZHENG Xingfei 1 1 胡晖 HU Hui 1 1 瞿桢 QU Zhen3 3 胡中立 HU Zhongli 1 1 武汉大学生命科学学院/植物发育生物学教育部重点实验室 武汉大学生命科学学院/植物发育生物学教育部重点实验室 College of Life Science and Technology,Chongqing University of Arts and Sciences College of Life Science and Technology,Chongqing University of Arts and Sciences 重庆文理学院生命科学与技术学院 重庆文理学院生命科学与技术学院 Hubei Shining Energy Plant Co.Ltd. Hubei Shining Energy Plant Co.Ltd. 湖北光芒能源植物有限公司 湖北光芒能源植物有限公司 College of Life Sciences,Wuhan University/Key Laboratory of Ministry of Education for Plant Developmental Biology College of Life Sciences,Wuhan University/Key Laboratory of Ministry of Education for Plant Developmental Biology 对41个来自于梁子岛上4个居群的五节芒（Miscanthus floridulus）进行相关序列扩增多态性（se-quence-related amplified polymorphism,SRAP）和简单重复序列区间标记（inter simple sequence repeat,ISSR）分析.利用POPGENE32version1.32软件计算多样性指数,采用NTSYS.2.10e计算软件,得到相似性系数,并用UPG-MA法进行聚类分析.结果显示6条ISSR多态性引物,共扩增出103条DNA条带,其中98条为多态性条带,占95.15%,样品之间的相似系数为0.67～0.95.6对SRAP引物,共扩增出173条DNA条带,其中147条为多态性条带,占84.97%,样品之间的相似系数为0.75～1.00.两种标记均表明五节芒具有较高的遗传多样性.分别以个体为单位及以群体为单位进行聚类分析,2种标记分别得到了相似但并不完全相同的个体聚类图及群体聚类图.研究结果表明五节芒的遗传多样性水平较高.SRAP与ISSR标记均适用于五节芒的遗传多样性分析. To study the genetic diversity of Miscanthus floridulus,forty-one individuals of M.floridulus from Liangzi Island were analyzed by sequence related amplified polymorphism（SRAP）and inter simple sequence repeats（ISSR）molecular markers.The data were analyzed by POPGENE32version1.32 and NTSYS.2.10esoftware and clustered by UPGMA method.Six ISSR primers amplified 103bands with 98（95.15%）polymorphic and six SRAP primer combination amplified 173bands with 147（84.97%） polymorphic.The genetic similarity coefficient was 0.67～0.95（by ISSRs）and 0.75～1.00（by SRAPs）.Both SRAP and ISSR analyses revealed a high level of genetic diversity in M.floridulus.By cluster analysis of the individuals or the populations,the dendrograms based on SRAP and ISSR markers were similar but not all the same.Different germplasms diversity of M.floridulus is high and SRAP and ISSR can be used in genetic diversity study of M.floridulus. 五节芒 相关序列扩增多态性 简单重复序列区间标记 遗传多样性 Miscanthus floridulus sequence related amplified polymorphism（SRAP） inter simple sequence repeats（ISSR） genetic diversity 10.14188/j.1671-8836.2010.05.004 Q943 2010-05-01 2021-04-01 5 56