高温胁迫下不同龙须菜品系抗氧化能力的比较

高温胁迫下不同龙须菜品系抗氧化能力的比较 Comparison of Antioxidant Activities of Different Strains of Gracilaria lemaneiformis under High-Temperature Stress 鹿宁 LU Ning1 1 first-author 臧晓南 ZANG Xiaonan1 1 张学成 ZHANG Xuecheng1 1 陈伟洲 CHEN Weizhou2 2 张璇 ZHANG Xuan1 1 顾颖慧 GU Yinghui 1 1 李清 LI Qing1 1 张璐 ZHANG Lu1 1 中国海洋大学海洋生命学院/海洋生物遗传育种教育部重点实验室 中国海洋大学海洋生命学院/海洋生物遗传育种教育部重点实验室 College of Marine Life Sciences,Ocean University of China/Key Laboratory of Marine Genetics and Breeding,Ministry of Education College of Marine Life Sciences,Ocean University of China/Key Laboratory of Marine Genetics and Breeding,Ministry of Education 汕头大学海洋生物研究所 汕头大学海洋生物研究所 Marine Biology Institute,Shantou University Marine Biology Institute,Shantou University 以龙须菜耐高温新材料07-2、龙须菜良种981及野生型龙须菜3个品系为材料,研究在不同温度条件下（24、28、32℃）其总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化物酶（POD）的活性变化,及抗坏血酸（维生素C）、维生素E、可溶性蛋白和丙二醛（MDA）的含量变化.结果表明:高温胁迫条件下,981和07-2的总抗氧化能力均高于野生型,28℃时981上升幅度比野生型高102.35%,07-2比野生型高18.86%;32℃时981比野生型上升幅度高17.87%,07-2则比野生型高73.52%.高温胁迫条件下,无论是酶促抗氧化系统还是非酶促抗氧化系统981和07-2都不同程度地优于野生型.可见,高温胁迫条件下981和07-2与野生型相比具有更强的清除活性氧的能力,表明抗氧化活性指标与龙须菜耐热性有相关性. To compare the antibioxidant activities of three strains of Gracilaria lemaneiformis,including new heat-resistant material 07-2,strain 981and wide-type,the variations of their total antioxidant capacity（T-AOC）,superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-PX）,peroxidase（POD）,Vc,Ve,soluble protein and maleic dialdehyd（MDA）under different high temperatures（24,28,32℃）were studied.The results showed that under heat stress the T-AOC of 07-2and 981were stronger than that of wide type.At 28℃,the T-AOC of 981increased 102.35% more than that of widetype,and the T-AOC of 07-2increased 18.86% more than that of wide-type.At 32℃,the T-AOC of 981 increased 17.87% more than that of wide-type,and the T-AOC of 07-2increased 73.52% more than that of wide-type.Under high temperature,no matter the enzymatic antioxidant activity or the non-enzymatic antioxidant activity of both 07-2and 981were better than that of wide type.These results indicate that the ability of scavenging ROS of 07-2and 981was higher than that of wide type under high temperature.The results also suggest that antioxidant system activity was responsible for the heat-resistant ability of Gracilaria lemaneiformis. 高温胁迫 龙须菜 抗氧化系统活性 high-temperature stress Gracilaria lemaneiformis antioxidant system activities 10.14188/j.1671-8836.2010.05.018 S917.3 国家高技术研究发展计划（863）重大项目（2006AA10A413）;教育部博士点基金新教师项目（20070423011）;广东省汕头市科技计划项目（2007-44）;经济海藻良种产业化技术体系研究与示范（200803052） 2010-05-01 2021-04-01 5 56