萤光素-Dy（Ⅲ）配合物与鲱鱼精DNA的作用方式

萤光素-Dy（Ⅲ）配合物与鲱鱼精DNA的作用方式 Interaction Mode Between Fluorescein-Dy（Ⅲ） Complex and Herring Sperm DNA 潘海专 PAN Haizhuan1 1 first-author 王兴明 WANG Xingming1 1 丁立生 DING Lisheng2 2 西南科技大学材料科学与工程学院 西南科技大学材料科学与工程学院 School of Materials Science and Engineering,Southwest University of Science and Technology School of Materials Science and Engineering,Southwest University of Science and Technology 中国科学院成都生物研究所 中国科学院成都生物研究所 Chengdu Institute of Biology,Chinese Academy of Sciences Chengdu Institute of Biology,Chinese Academy of Sciences 研究了Tris-HCl（pH7.40）缓冲溶液中萤光素（F）与稀土金属离子Dy（Ⅲ）形成的配合物Dy（Ⅲ）（F）3与鲱鱼精DNA的相互作用机制,实验测得Dy（Ⅲ）（F）3与鲱鱼精DNA的结合比为5:1,表观摩尔吸光系数ε=1.14×105L·mol-1·cm-1,Dy（Ⅲ）（F）3与鲱鱼精DNA的结合常数K14℃=1.78×104L·mol-1,14℃时ΔrHm=1.03×104J·mol-1,ΔrSm=1.17×102J·mol-1·K-1,ΔrGm=-2.34×104J·mol-1.Dy（Ⅲ）（F）3与鲱鱼精DNA主要以部分嵌插和沟渠结合方式发生作用,作用过程为熵驱动. The mechanism of the interaction of fluorescein-Dy（Ⅲ）complex with herring sperm DNA was investigated in Tris-HCl buffer（pH 7.40）.It is calculated that the binding ratio of nDy（Ⅲ）（F）3:nDNAis 5:1,and the apparent molar absorptivity is 1.14×105 L·mol-1·cm-1.The bonding constant between Dy（Ⅲ）（F）3and DNA is 1.78×104 L·mol-1（14℃）and the themodynamic functions areΔrHm=1.03×104 J·mol-1,ΔrSm=1.17×102 J·mol-1·K-1,ΔrGm=-2.34×104 J·mol-1 at 14℃.The results confirm that the interaction between Dy（Ⅲ）（F）3complex and DNA is a partial intercalation and groove interaction,and the reaction process is entropy favorable. 萤光素 Dy（Ⅲ） 配合物 鲱鱼精DNA 作用机制 fluorescein Dy（Ⅲ） complex herring sperm DNA interaction mode 10.14188/j.1671-8836.2010.05.020 R346 国家自然科学基金资助项目（30572254） 2010-05-01 2021-04-01 5 56