氯酚对乙酰胆碱酯酶的抑制作用及其动力学研究

氯酚对乙酰胆碱酯酶的抑制作用及其动力学研究 Inhibition Effect of Chlorophenols on Acetylcholinesterase and Its Kinetics 刘琼玉 LIU Qiongyu1 1 2 first-author 周培疆 ZHOU Peijiang1 1 陈岩 CHEN Yan1 1 2 武汉大学资源与环境科学学院/生物质资源化学与环境生物技术湖北省重点实验室 武汉大学资源与环境科学学院/生物质资源化学与环境生物技术湖北省重点实验室 College of Resources and Environmental Sciences,Wuhan University/Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory College of Resources and Environmental Sciences,Wuhan University/Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory 江汉大学化学与环境工程学院 江汉大学化学与环境工程学院 School of Chemistry and Environmental Engineering,Jianghan University School of Chemistry and Environmental Engineering,Jianghan University 研究了2-氯酚（2-CP）及2,4-二氯苯酚（2,4-DCP）对乙酰胆碱酯酶（AChE）活性的抑制作用及抑制动力学.结果表明,两种氯酚均能显著地抑制AChE的活性,2-CP及2,4-DCP对AChE酶活力的50%抑制浓度（IC50）分别为0.68,0.56mmol·L-1.2-CP及2,4-DCP对AChE的抑制作用类型为混合型的可逆抑制,2-CP对AChE的混合型抑制常数Ki,K′i分别为0.42,2.80mmol·L-1,2,4-DCP对AChE的混合型抑制常数Ki,K′i分别为0.37,0.70mmol·L-1,表明两种氯酚对游离酶的抑制作用均强于其对酶-底物复合物的抑制作用,且后者较前者对乙酰胆碱酯酶的抑制作用更强. The inhibition effect of ortho-chlorophenol（2-CP）and 2,4-dichlorophenol（2,4-DCP）on acetylcholinesterase（AChE）and its kinetics were studied by spectrophotometry.The results show that the activity of AChE can be inhibited obviously by 2-CP and 2,4-DCP,and the 50%inhibitory concentration（IC50）is 0.68mmol·L-1for 2-CP and 0.56mmol·L-1 for 2,4-DCP,respectively.Reversible inhibition of AChE by 2-CP and 2,4-DCP has been observed,and the type of reversible inhibition is mixed inhibition.The mixed inhibitory constants Kiand K’iare 0.42mmol·L-1 and 2.80mmol·L-1for 2-CP,and are 0.37 mmol·L-1 and 0.70mmol·L-1for 2,4-DCP,respectively.The inhibitory kinetic results indicate that the inhibition effect of chlorophenols on free enzyme is stronger than that on enzyme-substrate complex,and the inhibition effect of 2,4-DCP is stronger than that of 2-CP. 氯酚 乙酰胆碱酯酶 抑制作用 动力学 chlorophenols acetylcholinesterase inhibition effect kinetics 10.14188/j.1671-8836.2010.05.021 R99 国家高技术研究发展计划（863计划）资助项目（2007AA06Z418）;国家自然科学基金资助项目（20777058,20977070）;湖北省生物质资源化学与环境生物技术重点实验室（武汉大学）资助项目（HBRCEBL2008005） 2010-05-01 2021-04-01 5 56