乌药提取物的抗肿瘤及抗氧化活性

乌药提取物的抗肿瘤及抗氧化活性 Anticancer and Antioxidant Activity of Lindera strychnifolia Extracts 晏润纬 YAN Runwei1 1 2 first-author 彭小梅 PENG Xiaomei3 3 邹国林 ZOU Guolin2 2 南昌大学第一附属医院消化科 南昌大学第一附属医院消化科 Department of Gastroenterology,The First Affiliated Hospital of Nanchang University Department of Gastroenterology,The First Affiliated Hospital of Nanchang University 武汉大学生命科学学院/病毒学国家重点实验室 武汉大学生命科学学院/病毒学国家重点实验室 College of Life Sciences,Wuhan University/State Key Laboratory of Virology College of Life Sciences,Wuhan University/State Key Laboratory of Virology 江西省疾病预防控制中心 江西省疾病预防控制中心 Center for Disease Control and Prevention of Jiangxi Province Center for Disease Control and Prevention of Jiangxi Province 使用不同极性的有机溶剂分离得到了5种（石油醚、氯仿、乙酸乙酯、正丁醇、水）乌药提取物,并采用多种实验方法研究了其抗肿瘤及抗氧化活性.抗肿瘤活性研究结果显示:乌药石油醚萃取物对供试4种人癌细胞的细胞毒性最强;而HepG2细胞对乌药石油醚部位的细胞毒性最为敏感,半数抑制率（IC50）为（71.9±1.1）mg·L-1;乌药石油醚萃取物是通过诱发细胞凋亡来达到其体外抗HepG2作用的.抗氧化活性研究结果显示:乌药乙酸乙酯萃取物具有最强的体外抗氧化能力. Anticancer and antioxidant activities of 5 fractions（petroleum ether,chloroform,ethyl acetate,1-butanol,water） of ethanol extract of Lindera strychnifolia were investigated.The results of anticancer assay show that petroleum ether fraction has the strongest cytotoxic activity on 4 human cancer cell lines tested.HepG2（IC50:（71.9±1.1） mg·L-1） is most vulnerable to the cytotoxic effect of petroleum ether fraction.And the petroleum ether fraction induces apoptosis in HepG2.The results of antioxidant assay show that the ethyl acetate fraction exhibits strongest antioxidant activity among all the samples of Lindera strychnifolia. 乌药 提取物 抗肿瘤活性 抗氧化活性 Lindera strychnifolia extracts anticancer activity antioxidant activity 10.14188/j.1671-8836.2011.03.010 R285 国家自然科学基金资助项目（20873092） 2011-03-01 2021-04-01 3 57