对氯苯酚在酪氨酸酶-碳纳米管修饰电极上的电化学行为

对氯苯酚在酪氨酸酶-碳纳米管修饰电极上的电化学行为 Electrochemistry of p-Chlorophenol on a Tyrosinase-Carbon Nanotube Modified Electrode 刘华俊 LIU Huajun1 1 first-author 陈敏 CHEN Min1 1 刘慧宏 LIU Huihong2 2 襄樊学院化学工程与食品科学学院 襄樊学院化学工程与食品科学学院 School of Chemical Engineering and Food Science,Xiangfan University School of Chemical Engineering and Food Science,Xiangfan University 武汉纺织大学化学与化工学院 武汉纺织大学化学与化工学院 College of Chemistry and Chemical Engineering,Wuhan Textile University College of Chemistry and Chemical Engineering,Wuhan Textile University 研究了对氯苯酚在酪氨酸酶-碳纳米管修饰电极上的酶催化氧化和电化学还原过程.实验表明,酶催化反应和电化学反应均受溶液pH值的影响,酶催化反应为控制步骤.对氯苯酚的浓度在0.12 1 .11 mmol.L-1范围内,与电流呈线性关系,检出限为14μmol.L-1.酶催化反应的表观米氏常数Km=163μmol.L-1. The use of electrochemical biosensors has been shown to be a suitable alternative with respect to the conventional well-established analytical techniques for the monitoring of p-chlorophenol.The reactions of p-chlorophenol on a tyrosinase-carbon nanotube modified electrode were explored.The processes contain the oxidation of p-chlorophenol by enzyme catalysis and the electrochemical reduction of the product.The solution pH effects the reactions.The results reveal that the reaction of enzyme catalysis is the controlled step.The current and p-chlorophenol concentration is a linear relationship on the range of 0.12-1.11 mmol·L-1.The limit of detection is calculated to be 14 μmol·L-1.The Michaelis constant is estimated to be K m =163 μmol·L-1. 对氯苯酚 酪氨酸酶 碳纳米管 电化学 p-chlorophenol tyrosinase carbon nanotube electrochemistry 10.14188/j.1671-8836.2011.04.001 O646.54 湖北省自然科学基金资助项目（2008CDB014）;湖北省教育厅重大项目（Z20092501） 2011-04-01 2021-04-01 4 57