鱼腥草外用抗菌霜剂的制备及局部皮肤抗感染研究

鱼腥草外用抗菌霜剂的制备及局部皮肤抗感染研究 Preparation and Anti-Infective Activity of Houttuynia cordata Topical Cream 梁菊 LIANG Ju 1 first-author 陈姗 CHEN Shan 1 刘秋伟 LIU Qiuwei 1 池志娟 CHI Zhijuan 1 河南科技大学化工与制药学院 河南科技大学化工与制药学院 Chemical Engineering and Pharmaceutics College,Henan University of Science and Technology Chemical Engineering and Pharmaceutics College,Henan University of Science and Technology 以系统溶剂法分别制备了鱼腥草的石油醚提取物、氯仿提取物、乙酸乙酯提取物、正丁醇提取物和水提取物.各提取物的体外抑菌实验表明,鱼腥草的氯仿提取物对金黄色葡萄球菌、苏云金杆菌、大肠杆菌、枯草芽孢杆菌和白色葡萄球菌的生长具有很好的抑制作用.以该提取物为主要成分,制作了一种鱼腥草的外用抗菌霜剂.豚鼠皮肤刺激实验表明,该霜剂对皮肤无刺激性.豚鼠皮内注射金黄色葡萄球菌皮肤局部感染模型的建立及抗感染实验结果表明,该霜剂对金黄色葡萄球菌引起的皮肤局部感染有明显的治疗效果,每天给药3次,每次给药0.1 g（区域面积20 mm×30 mm）,7 d后皮肤红肿现象完全消失;局部皮肤体外微生物培养实验发现,注射部位皮肤已无病原菌感染. Five extracts of Houttuynia cordata was obtained using petroleum ether,chloroform,ethyl acetate,n-butanol and water as the solvents.The results of antibacterial experiments in vitro showed that chloroform fraction has strong antibacterial activity against Staphylococcus aureus,Bacillus thuringiensis,Escherichia coli,Bacillus subtilis,and Staphylococcus albus.A topical cream was prepared with chloroform extract as the main ingredient and was evaluated by acute skin irritancy in which the cream showed less irritant and safety for topical application.The guinea pig model of Staphylococcus aureus intradermal infection was then established and used for testing anti-infective activity of the cream.Skin redness and swelling disappeared after dermic administration 7 days with 0.1 g of the cream（20 mm×30 mm） three times a day.And the injected area has no bacteria survival by the bacterial culture of local skin in media.Those results hinted that Houttuynia cordata topical cream might be developed for potential clinical anti-infection drug. 鱼腥草 霜剂 抗菌活性 Houttuynia cordata cream antibacterial activity 10.14188/j.1671-8836.2011.04.009 R283;R285 河南省教育厅自然科学研究计划（2011A350001）资助;河南科技大学SRTP资助项目（2010） 2011-04-01 2021-04-01 4 57