MyoD gene was obtained using reverse transcription-polymerase chain reaction（RT-PCR） and rapid-amplification of cDNA end（RACE） method from yellow catfish（Pelteobagrus fulvidraco）.The bioinformatics of the gene and differential expression in female and male adult tissues were analyzed.The results show that MyoD ORF consists of 810 nucleotides encoding 269 amino acids

including a bHLH domain which is composed from 90th to 141th.The MyoD shows high homogeneity to that of channel catfish（93%） and other animals

suggesting MyoD is highly conserved through evolution.The real-time PCR analysis reveals that the expression level in muscle and stomach of male are higher than that of female’s significantly（p<0.01）

while the level in kidney and gill of female are higher than that of male’s significantly（p<0.05）.The expression difference which exists between female and male is probably one of important factors relating to the growth dissimilarity.