云南南部5种魔芋属植物居群遗传结构的ISSR分析

云南南部5种魔芋属植物居群遗传结构的ISSR分析 Population Genetic Structure of Five Amorphophallus Species from the South of Yunnan Province by Inter-Simple Sequences（ISSR） Markers 任盘宇 REN Panyu1 1 first-author 潘明清 PAN Mingqing2 2 中国科学院西双版纳热带植物园 中国科学院西双版纳热带植物园 Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 采用9个ISSR（inter-simple sequence repeat）分子标记对云南南部魔芋属植物5个物种（花魔芋,疣柄魔芋,攸乐魔芋,勐海魔芋和屏边魔芋）105份材料进行了居群遗传多样性分析.通过PCR扩增,9个ISSR引物从5个物种105份样品中共获得95条条带,其中多态性条带占条带总数的100%.每个位点平均等位基因数2.00,有效等位基因数1.40,平均Nei’s基因多样性指数0.255 2,Shannon’s信息多样性指数为0.401 2.在这5个魔芋属植物物种中,花魔芋居群内遗传多样性水平相对较低.居群水平上的UPGMA聚类表明这5个物种可被分为明显的两大组,花魔芋为一组,其余4种构成一组,其中屏边魔芋与疣柄魔芋遗传距离最近.个体水平上的聚类则显示,花魔芋和疣柄魔芋的个体各自聚在一起,其余3种的个体较为分散.本研究结果还认为人工栽培利用的方式对魔芋属植物遗传多样性可能存在一定影响. The genetic diversity of five Amorphophallus species（A.konjac,A.dubius,A.yuloensis,A.kachinensis,A.pingbianensis） from the south of Yunnan province were studied with inter-simple sequence（ISSR） markers.A total of 105 individuals were included in this study.By using nine ISSR primers,a total of 95 fragments were amplified,of which 95 were polymorphic.The observed number of alleles（NA） was 2.00,and the effective number of alleles was 1.40,the Nei’s gene diversity was 0.255 2,and the Shannon’s information index was 0.401 2.Among the five studied species,the lowest genetic diversity was found in A.konjac.The UPGMA cluster of the five species showed that five Amorphophallus species could be divided into two groups.The first group included the A.konjac,and the another group included A.dubius,A.yuloensis,A.kachinensis and A.pingbianensis.The genetic distance between A.pingbianensis and A.dubius was the smallest.At the individual level,cluster analysis demonstrated that the individuals of A.konjac and A.dubius were clustered into their corresponding species,and the rest of individuals did not cluster into their corresponding species.This results also suggested that the artificial cultivation might have an important role in reducing the genetic diversity of Amorphophallus species. 魔芋属 ISSR 遗传多样性 云南省 Amorphophallus ISSR genetic diversity Yunan Province 10.14188/j.1671-8836.2013.01.019 S632.9 神农架地区本底资源综合调查项目资助（20125nj004） 2013-01-01 2021-04-01 1 59