Gd（Ⅲ）-EBBR配合物与鲱鱼精DNA的作用方式

Gd（Ⅲ）-EBBR配合物与鲱鱼精DNA的作用方式 Interaction Mode between Gd（Ⅲ）-EBBR Complex and Herring Sperm DNA 白菊 BAI Ju 1 first-author 刘爱 LIU Ai 1 王兴明 WANG Xingming 1 西南科技大学材料科学与工程学院 西南科技大学材料科学与工程学院 School of Materials Science and Engineering,Southwest University of Science and Technology School of Materials Science and Engineering,Southwest University of Science and Technology 在pH 7.4的tris-HCl溶液中,Gd（Ⅲ）与铬蓝黑R（EBBR）生成1∶3的配合物,配合物与鲱鱼精DNA形成5∶1的复合物Gd（Ⅲ）（EBBR）3-DNA.通过双倒数法和热力学公式计算得到复合物Gd（Ⅲ）（EBBR）3-DNA在不同温度下的结合常数和其他的热力学常数.结合探针法、碱基模拟、Scatchard法和黏度法表明Gd（Ⅲ）（EBBR）3与DNA是通过嵌插作用和沟槽作用形成复合物Gd（Ⅲ）（EBBR）3-DNA.配合物Gd（Ⅲ）（EBBR）3与鲱鱼精DNA形成复合物的过程是由焓和熵共同驱动. The binding ratio between Gd（Ⅲ）and eriochrome blue black R（EBBR）in tris-HCl solution（pH 7.4） was determined as nGd（Ⅲ） ∶nEBBR =1∶3,and the binding ratio between Gd（Ⅲ）（EBBR） 3 and DNA was determined as 5 ∶1.By double reciprocal method and thermodynamic formulae,the binding constants of Gd（Ⅲ）（EBBR） 3-DNA at different temperature and other thermodynamic constants were confirmed.Combined with probe method,basic group simulation,Scatchard method and viscosity method,the results suggest that the interaction mode between Gd（Ⅲ）（EBBR） 3 complex and herring sperm DNA are intercalation binding and groove binding.Entropy and enthalpy are the primary driving power of the interaction of Gd（Ⅲ）（EBBR） 3 complex and herring sperm DNA. 鲱鱼精DNA Gd（Ⅲ）-EBBR配合物 嵌插作用 Scatchard法 herring sperm DNA Gd（Ⅲ）-EBBR complex intercalation binding Scatchard method 10.14188/j.1671-8836.2014.01.006 O641.4 国家自然科学基金资助项目（30572254） 2014-01-01 2021-04-01 1 60