气阀控微流控芯片中微液滴的研究

气阀控微流控芯片中微液滴的研究 Research on Micro-Droplet In Microfluidic Chip Based Pneumatic Valve 张蒙 ZHANG Meng 1 first-author 郭志霄 GUO Zhixiao 1 赵兴中 ZHAO Xingzhong 1 武汉大学物理科学与技术学院 武汉大学物理科学与技术学院 School of Physics and Technology,Wuhan University School of Physics and Technology,Wuhan University 为了实现微液滴的可控制备和应用,设计和制作了一种集成有微气阀的双层微流控芯片,在该芯片上产生体积可控的水相微液滴,并对微液滴进行了操控.基于该平台研究了β-半乳糖苷酶（β-Gal）的酶促反应,制备了含有β-Gal及其底物乳糖RGP的微液滴并使之融合,在混合均匀后将其捕获,原位观察融合前后微液滴荧光强度的变化.并在底物溶液中加入酶抑制剂（DTPA）,研究了DTPA对于酶活性的抑制作用. To study the preparation and application of micro-droplets,a double layer pneumatic valve-based microfluidic chip was fabricated in this article,size controllable aqueous droplets were produced and manipulated on the chip.On this platform,a enzyme catalysis reaction was studied,β-D-galactosidase（β-Gal）and its substrate resorufinD-galactopyranoside（RGP）were encapsulated into droplets individually,and the droplets was fused,well-mixed and trapped for fluorescence assay.Then,enzyme inhibitor（DTPA）was added into the RGP solution to investigate the inhibition effect of the DTPA to the enzyme activity. 微流控 微气阀 微液滴 融合 microfluidic pneumatic valve micro-droplet fusion 10.14188/j.1671-8836.2014.02.010 TN492 国家自然科学基金资助项目（10804087） 2014-02-01 2021-04-01 2 60