多巴胺修饰超小Gd₂O₃纳米粒子的制备及MRI评价

多巴胺修饰超小Gd 2 O 3 纳米粒子的制备及MRI评价 Preparation and MRI Evaluation of Dopamine-Modified Ultra-Small Gd 2 O 3 Nanoparticles 郝晶 HAO Jing 1 first-author 黄进 HUANG Jin 1 黄海涛 HUANG Haitao 2 武汉理工大学化学工程学院 武汉理工大学化学工程学院 College of Chemical Engineering,Wuhan University of Technology College of Chemical Engineering,Wuhan University of Technology 华中科技大学同济医学院协和医院 华中科技大学同济医学院协和医院 Union Hospital,Tongji Medical College,Huazhong University of Science and Technology Union Hospital,Tongji Medical College,Huazhong University of Science and Technology 将具有邻苯二酚结构的多巴胺用于超小Gd2O3纳米粒子的表面修饰,通过控制反应时间,制得适用于磁共振成像（MRI）增强对比度的造影剂.通过透射电镜（TEM）,红外光谱（FTIR）,紫外-可见吸收光谱（UV-vis）及热重分析（TGA）测试表征了多巴胺修饰Gd2O3纳米粒子的形貌、结构及组成.结果表明:平均粒径为3.7nm的Gd2O3纳米粒子经多巴胺修饰后,由于多巴胺分子中苯环的疏水性导致了一定程度的聚集;反应12h和24h对应的多巴胺修饰的超小Gd2O3纳米粒子中有机物的组成分别为34.7%和42.6%.MRI造影结果显示,多巴胺表面修饰的超小Gd2O3纳米粒子有效地增强了对比亮度,且弛豫效率和信号强度在一定浓度范围内与造影剂浓度呈正相关;反应较长时间（24h）对应的多巴胺修饰的超小Gd2O3纳米粒子的弛豫效率较高,对应的造影增强效果更好. The dopamine with a catechol structure was used to modify ultra-small Gd2O3nanoparticles（the diameter was lower than 5nm）,which produced the positive contrast enhanced agents for magnetic resonance imaging（MRI）by regulating the reaction time.The morphology,structure and composition of the as-prepared Gd2O3nanoparticles were characterized by transmission electron microscope（TEM）,Fourier transform infrared（FT-IR）spectroscopy,UV-vis spectroscopy and thermogravimetric analysis（TGA）.By the TEM observation,the Gd2O3nanoparticles with a mean diameter of ca.3.7nm tended to aggregate because of the hydrophobic benzene ring in dopamine.At the same time,the TGA results showed the dopamine-modified Gd2O3nanoparticles for the reaction time of 12and 24hours had the weight percentage of organic components are 34.7% and 42.6%,respectively.Moreover,the T1-weighted MRI-related measurements were carried out for the dopamine-modified Gd2O3nanoparticles.The results showed that the dopamine-modified Gd2O3nanoparticles effectively enhanced the contrast brightness,and the relaxation rates and signal intensity were positively correlated with the concentration of the contrast agent.A longer reaction time（24hours）for the surface modification of dopamine toward ultra-small Gd2O3nanoparticles contributed to higher relaxivity and better contrast enhancement effect thereof. 超小Gd2O3纳米粒子 多巴胺 表面修饰 T1加权MRI增强 ultra-small Gd2O3nanoparticles dopamine surface modification enhanced T1-weighted MRI 10.14188/j.1671-8836.2014.04.019 TQ421.7 科技部国家国际科技合作专项项目（2013DFG32340） 2014-04-01 2021-04-01 4 60