草果挥发油对肝癌H₂₂荷瘤小鼠的抑瘤作用

草果挥发油对肝癌H 22 荷瘤小鼠的抑瘤作用 Anti-Tumor Effect of the Essential Oil from Amomum tsao-ko on H 22 Tumor Bearing Mice 张琪 ZHANG Qi 1 2 first-author 杨扬 YANG Yang 2 3 桂林医学院基础医学院 桂林医学院基础医学院 Department of Basic Medicine,Guilin Medical University Department of Basic Medicine,Guilin Medical University 广西肝脏损伤与修复分子医学重点实验室 广西肝脏损伤与修复分子医学重点实验室 Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair 桂林医学院药学院 桂林医学院药学院 Department of Pharmacy,Guilin Medical University Department of Pharmacy,Guilin Medical University 建立肝癌H22荷瘤小鼠模型,探讨了草果挥发油对肿瘤质量、胸腺指数和脾脏指数的影响,采用HE染色法观察肿瘤细胞形态变化,免疫组化法检测肿瘤细胞凋亡相关蛋白Bax和Bcl-2的表达.结果发现,草果挥发油各剂量组均能明显抑制肿瘤生长（p<0.05）;高剂量组（300 mg·kg-1）有些微毒副作用,各剂量组胸腺指数和脾脏指数较环磷酰胺（CTX）组有明显升高（p<0.05）;推测草果挥发油能够抑制H22荷瘤小鼠肿瘤细胞生长的机制是通过上调Bax蛋白表达,下调Bcl-2蛋白表达来诱导细胞凋亡. The anti-tumor effects of essential oilfrom Amomum tsao-ko（ EOAT） in vivo was tested with the H22 tumor bearing mice model. The model mice were divided into five groups: model group,CTX group（ 20 mg·kg- 1）,and high-,middle- and low-dose（ 75,150,300 mg·kg- 1） EOAT groups. The tissues of tumor,thymus,as well as spleen were dissected and weighted. The tumor inhibition rate,thymus index and spleen index were calculated. The morphological changes of tumor cells were observed by HE staining. The expression of apoptosis related protein Bax and Bcl-2 was detected using immunohistochemistry. As a result,all doses of EOAT significantly inhibit tumor growth on H22mice（ p < 0. 05）. However,the high dose of EOAT has slightly side-effects. The thymus and spleen indexes of all EOAT groups increased significantly（ p <0. 05） compared with those of CTX group. EOAT could increase the expression of Bax,reduce the expression of Bcl-2. In a conclusion,EOAT could inhibit the growth of H22 tumor cell. The mechanism might be related to raise the expression of Bax and suppress the expression of Bcl-2 to induce apoptosis. 草果挥发油 肝癌H22细胞 抗肿瘤 细胞凋亡 Bax蛋白 Bcl-2蛋白 essential oilfrom Amomum tsao-ko hepatoma carcinoma cell H22 anti-tumor apoptosis Bax Bcl-2 10.14188/j.1671-8836.2015.02.013 R285.5 广西自然科学基金青年基金项目（2011GXNSFB018087）;广西教育厅科研项目（201010LX341）;桂林医学院青年教师科研启动基金项目资助 2015-02-01 2021-03-31 2 61