CdSeS合金量子点在阿霉素定量分析中的应用

CdSeS合金量子点在阿霉素定量分析中的应用 Application of Alloyed CdSeS Quantum Dots in Quantitative Determination of Doxorubicin 谢程立 XIE Chengli 1 first-author 曹启花 CAO Qihua 1 肖玲 XIAO Ling 1 彭善枝 PENG Shanzhi 1 黄祖螟 HUANG Zuming 1 武汉大学资源与环境科学学院/湖北省生物质资源化学和环境生物技术重点实验室 武汉大学资源与环境科学学院/湖北省生物质资源化学和环境生物技术重点实验室 School of Resource and Environmental Sciences,Wuhan University/Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory School of Resource and Environmental Sciences,Wuhan University/Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory 以巯基丙酸为稳定剂和硫源,采用水热法制备了CdSeS合金量子点,并将其应用于阿霉素（DOX）的定量分析.结果表明,DOX对CdSeS合金量子点有荧光猝灭作用,DOX分析的线性范围及检出限与CdSeS的浓度有关.在pH值为7.5的Tris-HCl缓冲溶液中,量子点浓度（以Cd2+计）为0.1,0.3,0.5 mmol·L-1时,对应的DOX分析线性范围分别为0.029 1 2,0.032 2 0,0.037 4 0mg·L-1,检出限分别为8.9,9.6,11.2μg·L-1.一定量的Cu2+和Fe3+会干扰DOX的测定.去除干扰物质后,对实际水样品进行分析,加标回收率为99% 1 02%,相对标准偏差小于1.4%. With mercaptopropionic acid as the stabilizing agent and sulfur precursor,the alloyed CdSeS quantum dots were prepared via hydrothermal method.A assay of doxorubicin（DOX）was proposed based on the interaction of as-prepared alloyed CdSeS quantum dots with DOX.The fluorescence signals of alloyed CdSeS quantum dots could be quenched effectively by DOX.The linear range and limit of detection for DOX depend on the concentration of alloyed CdSeS quantum dots.In the Tris-HCl buffer solution at pH 7.5,with 0.1,0.3,0.5mmol·L-1 CdSeS aqueous solution,the linear range varies in the DOX concentration range of 0.029-12,0.032-20,and 0.037-40mg·L-1;the calculated limits of detection are 8.9,9.6,and 11.2μg·L-1,respectively.A certain amount of Cu2+and Fe2+would interfere with the determination of DOX.The contents of DOX in real water sample were determined with the recovery rates of 99%-102% and the relative standard deviation below 1.4% after the separation of foreign substances. 合金量子点 阿霉素 荧光分析 alloyed quantum dot doxorubicin fluorescence analysis 10.14188/j.1671-8836.2015.03.005 X832;O657.3 湖北省自然科学基金（2014CFB728）资助项目 2015-03-01 2021-03-31 3 61