金银花蕾提取物及其3-咖啡酰奎宁酸抗乙肝病毒活性

杨邱; 李洁媚; 万浩强; 葛岚岚; 曾小斌; 彭树松

doi:10.14188/j.1671-8836.2019.04.005

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金银花蕾提取物及其3-咖啡酰奎宁酸抗乙肝病毒活性

药用植物与人类健康 | 更新时间：2023-08-02

- 金银花蕾提取物及其3-咖啡酰奎宁酸抗乙肝病毒活性
- Anti-HBV Activities of Extracts and 3-Caffeoylquinic Acid from Lonicera japonica Flower Buds
- 武汉大学学报（理学版） 2019年65卷第4期页码：352-356
- 作者机构：
  
  1.暨南大学第二临床学院深圳市人民医院消化内科，广东深圳 518120
  2.暨南大学第二临床学院深圳市人民医院龙华分院中心实验室，广东深圳 518120
  2.暨南大学第二临床学院深圳市人民医院龙华分院病理科，广东深圳 518120
- 作者简介：
  
  杨邱，女，副主任医师，硕士，现从事消化道疾病研究。E-mail：yangqiu66@126.com
  E-mail：shusongpeng@163.com
- 基金信息：
  
  广东省自然科学基金(2017A030313659)
- DOI：10.14188/j.1671-8836.2019.04.005
  中图分类号：
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杨邱, 李洁媚, 万浩强, 等. 金银花蕾提取物及其3-咖啡酰奎宁酸抗乙肝病毒活性[J]. 武汉大学学报（理学版）, 2019,65(4):352-356.

YANG Qiu, LI Jiemei, WAN Haoqiang, et al. Anti-HBV Activities of Extracts and 3-Caffeoylquinic Acid from Lonicera japonica Flower Buds[J]. J Wuhan Univ （Nat Sci Ed）, 2019,65(4):352-356.
杨邱, 李洁媚, 万浩强, 等. 金银花蕾提取物及其3-咖啡酰奎宁酸抗乙肝病毒活性[J]. 武汉大学学报（理学版）, 2019,65(4):352-356. DOI： 10.14188/j.1671-8836.2019.04.005.

YANG Qiu, LI Jiemei, WAN Haoqiang, et al. Anti-HBV Activities of Extracts and 3-Caffeoylquinic Acid from Lonicera japonica Flower Buds[J]. J Wuhan Univ （Nat Sci Ed）, 2019,65(4):352-356. DOI： 10.14188/j.1671-8836.2019.04.005.

摘要

以HepG2.2.15细胞为乙肝病毒（HBV）感染的体外实验模型，采用MTT法测定实验组细胞的活力，采用酶联免疫吸附试验（ELISA）测定实验组HepG2.2.15细胞上清液中乙肝病毒表面抗原（HBsAg）、e抗原（HBeAg）的含量，采用实时荧光定量PCR法检测HepG2.2.15细胞上清液HBV DNA水平，研究金银花蕾乙醇提取物（LA）、乙酸乙酯部位（LE）及活性成分3-咖啡酰奎宁酸体外抗乙肝病毒的作用。研究结果显示：LA、LE及3-咖啡酰奎宁酸对HepG2.2.15细胞的生长增殖均无显著抑制作用；LA和LE作用8 d可抑制HBeAg分泌；100 μg/mL 3-咖啡酰奎宁酸对HepG2.2.15细胞上清液中HBsAg、HBeAg分泌及DNA复制均具有显著的抑制作用。上述结果表明，金银花中的活性成分3-咖啡酰奎宁酸具有显著的抗HBV活性。

Abstract

HepG2.2.15 cells were used as a model for hepatitis B virus （HBV） infection ,in vitro., MTT assays were used to determine the viabilities of HepG2.2.15 cells in different experimental groups. In order to study the effects of ethanol extracts and 3-caffeoylquinic acid from ,Lonicera japonica, flower buds against HBV ,in vitro, enzyme-linked immunesorbent assays (ELISA) were applied to quantify the contents of HBsAg and HBeAg in the supernatant of each experimental group, and real-time PCR was applied to quantify the HBV DNA levels. The results indicate that the ethanol extract (LA), ethyl acetate fraction (LE) and 3-caffeoylquinic acid have no inhibitory effects on the viabilities of HepG2.2.15 cells; LA and LE can inhibit the secretion of HBeAg after 8-day treatment; 3-caffeoylquinic acid can inhibit the secretion of HBsAg, HBeAg and the replication of HBV DNA in HepG2.2.15 cells at the concentration of 100 μg/mL. In summary, the active ingredient 3-caffeoylquinic acid exerts prominent activity against HBV ,in vitro,.

关键词

金银花蕾乙肝病毒HepG2.2.153-咖啡酰奎宁酸

Keywords

Lonicera japonica flower budshepatitis B virus （HBV）HepG2.2.153-caffeoylquinic acid

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