NaCl对水稻谷氨酰胺合成酶活性及同工酶的影响

NaCl对水稻谷氨酰胺合成酶活性及同工酶的影响 Effect of NaCl Stress on Activity and Isozymes of Glutamine Synthetase in Rice Plants 李常健 1 2 3 first-author 林清华 1 2 3 张楚富 1 2 3 李泽松 1 2 3 彭进 1 2 3 朱英国 1 2 3 PengShaobing 1 2 3 BennettJohn 1 2 3 武汉大学生命科学学院,武汉,430072 武汉大学生命科学学院,武汉,430072 InternationalRiceResearchInstitute!.O.Box,P,3127 InternationalRiceResearchInstitute!.O.Box,P,3127 MakatiCity,1271 MakatiCity,1271 在营养液中 Ｎａ Ｃｌ的胁迫下，水稻根和叶的谷氨酰胺合成酶（ Ｇ Ｓ）的活性降低，但叶的 Ｇ Ｓ活性对 Ｎａ Ｃｌ浓度的敏感性大于根根和叶中的 Ｇ Ｓ活性变化与根和叶的平均质量和可溶性蛋白水平的变化是一致的 Ｎａｔｉｖｅ Ｐ Ａ Ｇ Ｅ活性染色以及 Ｉｍ ｍ ｕｎｏｂｌｏｔｔｉｎｇ 检测表明，根和叶的 Ｇ Ｓ活性的降低主要是由于 Ｇ Ｓｒｂ 和 Ｇ Ｓ２的活性及其相应蛋白质水平降低所致 The activity of glutamine synthetase （GS） in rice roots and leaves was decreased under NaCl stress in nutrient solution, but the activity of GS in Leaves was more sensitive to NaCl stress than that in roots.The change of GS activity in rice roots and leaves was consistent with the change of the biomass and soluble proteins in rice roots and leaves. The Determination of GS by Native PAGE, activity staining,and immunoblotting showed that the decline of GS activity of rice roots and leaves was mainly due to the reduction of the activity and protein levels of GSrb in roots and GS2 in leaves, respectively. 谷氨酰胺合成酶 同工酶 水稻 NaCl glutamine synthetase isozyme rice NaCl Q946.5r# 国家自然科学基金,湖北省自然科学基金,国际水稻研究所资助项目 1999-04-01 2021-04-01 4